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Absolute Quantitation of SARS-CoV-2 with Combinati Absolute Q dPCR Platform

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Learn about Absolute Q’s capability to perform 1-step RT-dPCR on a rare target dilution series by detecting as few as 5 viral RNA targets amongst a high background of human genomic DNA.

Application Highlights

  • 1st demonstration of a true 1-step RT-PCR on a digital PCR platform in under 90 minutes
  • Low limit of detection for viral load quantification down to 5 target copies per reaction
  • Potential to enable more efficient quantification of viral targets with up to 4-color multiplexing

The rapid outbreak of COVID-19 originating in Wuhan, China has mobilized unprecedented response to the pandemic across the globe. Numerous diagnostic tests have been deployed to aid in control of disease spread. However, the viral load profile from suspected exposure to symptom onset to complete recovery has yet to be fully characterized. Digital PCR (dPCR) technologies have demonstrated improved sensitivity and accuracy in positive SARS-CoV-2 detection, especially in low viral load samples that were reported to be negative using standard clinically approved RT-PCR methods.

This study highlights the Absolute Q’s capability to perform 1-step RT-dPCR for the quantitation of SARS-CoV-2 by detecting as few as 5 viral RNA targets among a high background of human genomic DNA. Additionally, we highlight the platform’s multiplexing capability using an in-house 3-color assay for the detection of the N1 and N2 SARS-CoV-2 viral targets alongside the RNaseP human control.

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Results of 3-color assay for SARS-CoV-2 detection on the Combinati Absolute Q across a dynamic range of input material consisting of 1000, 100, 5 and 0 copies of RNA targets – all among a high background of human genomic DNA (33ng or 10,000 copies).

Combinat’s Absolute Q platform is for research use only and has not been validated for use in diagnostic procedures.

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Additional Resources

SARs

SARS-CoV-2 Reference Material Quantification on the Absolute Q dPCR Platform

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Multiplexed-1

Multiplexed 1-step RT-dPCR for quantitation of ultra-rare SARS-CoV-2 viral targets

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