The rapid outbreak of COVID-19 originating in Wuhan, China has mobilized unprecedented response to the pandemic across the globe. Numerous diagnostic tests have been deployed to aid in control of disease spread. However, the viral load profile from suspected exposure to symptom onset to complete recovery has yet to be fully characterized. Digital PCR (dPCR) technologies have demonstrated improved sensitivity and accuracy in positive SARS-CoV-2 detection, especially in low viral load samples that were reported to be negative using standard clinically approved RT-PCR methods.
This study highlights the Absolute Q’s capability to perform 1-step RT-dPCR for the quantitation of SARS-CoV-2 by detecting as few as 5 viral RNA targets among a high background of human genomic DNA. Additionally, we highlight the platform’s multiplexing capability using an in-house 3-color assay for the detection of the N1 and N2 SARS-CoV-2 viral targets alongside the RNaseP human control.
Results of 3-color assay for SARS-CoV-2 detection on the Combinati Absolute Q across a dynamic range of input material consisting of 1000, 100, 5 and 0 copies of RNA targets – all among a high background of human genomic DNA (33ng or 10,000 copies).